It is recommended that the confident deposition results, measurement methods and sampling approaches must be standardized for UAV sprayers according to the field conditions and controlled within artificial assessments. It can be concluded that variation in recorded depositing is due to the sampling material. The results also showed the water sensitive paper recorded an 80.3% higher deposition than that of the glass strip at zero position during the driving flight height 2 m and flight speed 2 ms−1 (T1 treatment). The results showed that the water-sensitive paper recorded the droplet deposition in the target zone with a range from 0.049 to 4.866 µLcm−2, whereas the glass strip recorded from 0.11 to 0.793 µLcm−2. The results showed a variation in the estimation of the spray deposits among the two applied sampling methods. In this study, two sampling approaches (water-sensitive paper, and glass strip collectors) were compared to analyze spray deposition in target and off-target zones. There are various approaches available for sampling spray deposition and drift, during the evaluation of ground sprayers used for the UAV sprayer assessment, under field conditions. A decisive stage in the measurement of spray deposition and drift is selecting an appropriate sampling approach under field conditions. Target and off-target spray depositions determine the spray’s effectiveness and impact on the environment.